Bordetella Lyo PCR Kit

Product Description

The Hola Bordetella Lyo PCR Kit enables highly sensitive detection and differentiation of Bordetella pertussis and Bordetella parapertussis using real-time PCR. The assay targets the IS481 and IS1001 multicopy insertion sequences, ensuring high sensitivity, while the ptxA gene encoding the pertussis toxin provides an additional specific marker for B. pertussis identification.

This three-target approach allows for accurate identification of whooping cough–causing pathogens, supporting faster diagnostics and outbreak control. The lyophilized format eliminates cold chain requirements, simplifies workflows, and ensures long-term stability — making it ideal for both centralized laboratories and decentralized or resource-limited settings.

Technical Specifications

• Single-reaction well format (PCR strips)

• Compatible instruments: Bio-Rad CFX96 / CFX Opus, Hola MDX01, Hola MDX08

• For research use only (RUO)

• Endogenous internal control

• Three-channel multiplex PCR

• Sample type: Sputum, nasal cavity, and nasopharyngeal samples

• Specificity: 100%

• Sensitivity (LoD):

  • IS481 = 0.1 copies/μl

  • IS1001 = 0.7 copies/μl

  • ptxA-Pr = 5.9 copies/μl

• Storage and transport: +15 °C to +25 °C (room temperature)

Each Kit Contains

• Lyophilized MasterMix in pre-aliquoted strip wells

• Lyophilized Positive Control

• Negative Control