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Bordetella Lyo PCR Kit

Producer: Hola Biolabs s.r.o.
Product code: HB007T

Lyophilized Real-Time PCR kit for the detection of Bordetella pertussis and Bordetella parapertussis.

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Product description

The Hola Bordetella Lyo PCR Kit enables highly sensitive detection of Bordetella pertussis and Bordetella parapertussis bacteria using IS481 and IS1001 multicopy sequences. For unambiguous determination, one of the targets of the reaction is also the gene for the pertussis toxin PtxA.

Technical specifications

  • Endogenous internal control
  • Three-channel multiplex PCR
  • Sample type: sputum, upper respiratory tract samples (nasal cavity, nasopharynx)
  • Specificity: 100%
  • Sensitivity (LoD): 0.1 copies/μL (IS481), 0.7 copies/μL (IS1001), 5.9 copies/μL (ptxA-Pr)
  • Recommended instruments: CFX96/CFX Opus (Bio-Rad), MIC (BMC)
  • In vitro diagnostic medical device (IVD)
  • Storage and transport at a temperature of +15 °C to +25 °C

The kit contains

  • 1x Lyophilized MasterMix
  • 1x Lyophilized Positive control
  • 1x Negative control
  • 1x Reconstitution buffer
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